The 2-color fluorescence microscope body combines two CMOS sensors for simultaneous imaging of two different fluorophores.
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The 2-color fluorescence microscope body combines two CMOS sensors for simultaneous imaging of two different fluorophores. Due to the chromatic aberrations of GRIN lenses, the position of each image sensor is adjusted to correct the chromatic shift and image the same object plane in both colors. As the chromatic shift is related to the length of the GRIN lens, the correction is valid for one specific length of GRIN lens. For now, Green (GFP like) + Red (RFP like) systems are offered: two systems optimized for Snap-in Imaging Cannulas type D and type V, and one system optimized for surface imaging.
• The Electrical Cable for 2-color Fluorescence Microscope Bodies is required for the use of this device (see Related Products).
• The optical fiber length is adjusted to fit the desired electrical cable length.
• Every microscope body comes with a protective cap.
|Field of view||Model S : 730 µm x 730 µm (600 pixels x 600 pixels) Model L : 320 µm x 320 µm (600 pixels x 600 pixels)|
|Lens magnification||Model S: 3x Model L: 6.5x|
|Objective lens NA||0.5|
|Excitation wavelength||465 nm and 561 nm|
|Collection spectrum||520/35 nm and 615/45 nm|
|Frame rate||45 fps|
|Computer requirements||Intel Core-i7, 8BG RAM, Gigabit Ethernet and Jumbo frame compatible|
|Dimensions without cannula & cables (W x L x H)||18 mm x 17 mm 9.5 mm|
Application note for the surgical implantation & installation of the 2-color fluorescence microscopeDownload (3.62M)
For deeper brain regions (150 µm to 8 mm depth) use the Snap-in Imaging Cannula Model L with image guiding gradient-index rod lens that brings the image from inside the brain to the skull surface. Snap-in Imaging Cannulas are compatible with all Fluorescence Microscope Bodies of the corresponding model.
For areas near the brain surface (<150 µm depth) use the Snap-in Imaging Cannula Model S. Snap-in Imaging Cannulas are compatible with all Fluorescence Microscope Bodies of the corresponding model.
Our vast product line allows you to assemble systems for miniaturized fluorescence microscopy, fiber pho-tometry and optogenetically synchronized electrophysiology. The Doric Neuroscience Studio is an intuitive software which allows you to control the hardware and acquire all the required data simultaneously.Downloads DORIC STUDIO V18.104.22.168...
2-color Fluorescence Microscope Bodies are connectorized in order to allow for more flexibility.
The 2-color Fluorescence Microscope Driver is compatible with the 2-color Fluorescence Microscope Bodies.
A specific pigtailed microscope holder has been designed to fit the 2-color fluorescence microscope bodies. This holder can be used to image the brain during the implantation of the cannula or during experiments requiring a head-fixed in vivo configuration.