The Optogenetically Synchronized Fluorescence Microscope or OSFM, combines fluorescence imaging and optogenetic stimulation/inhibition capabilities within the miniature fluorescence microscope.
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The Optogenetically Synchronized Fluorescence Microscope or OSFM, combines fluorescence imaging and optogenetic stimulation/inhibition capabilities within the miniature fluorescence microscope. It can be used for freely-moving or head-fixed configurations. To avoid cross talk between optogenetic stimulation and fluorescence imaging, the OSFM hardware provides for at least two distinct spectral bands for light activation or fluorophore excitation (like blue and yellow) and at least two distinct spectral bands for imaging of fluorophores (like green and red). Either channel, blue-green or yellow-red can be used for opsin activation/inhibition or for calcium indicator excitation and imaging. As the field of opsins and calcium indicators is very dynamic, those spectral bands can be tailored to specs. For now, GCaMP6 + NpHR3.0 and RCaMP2 + ChR2 microscope versions are available.
• The ultralight fiberglass jacket is lighter and more flexible, the lightweight metal jacket is more robust but heavier.
• The standard electrical cable length is 1000 mm. Other values on request (up to 3000 mm).
• The optical fiber length is adjusted to fit the desired electrical cable length.
• Every microscope body comes with a protective cap.
Deep brain imaging of calcium signals at 10 fps in neurons expressing GCaMP6 in a freely moving mouse using a Doric Optogenetically Synchronized Fluorescence Microscope and a Snap-in Imaging Cannula Model L type V (up to 6.0 mm deep). Courtesy of Adam Packer, Michael Häusser lab, UCL. On the left is the raw movie. On the right is the processed ΔF/F0, computed with our free Image Analysis module.
Under is the ΔF/F0 analysis of the video. The image shows 25 traces (ΔF/F0 up to 10%) of the >50 cells found in the movie.
The following video demonstrates the implantation of a snap-in cannula and the installation of a miniaturized fluorescence microscope.
|Field of view||Model S : 700 µm x 700 µm (630 pixels x 630 pixels) Model L : 350 µm x 350 µm (630 pixels x 630 pixels)|
|Lens magnification||Model S: 3.3x Model L: 6x|
|Objective lens NA||0.5|
|Excitation wavelength||445/62 nm or 549/15 nm|
|Collection spectrum||525/40 nm or 609/57 nm|
|Opsin activation||616/76 nm or Compatible with 450 nm, 473 nm, 488 nm|
|Frame rate||45 fps|
|Computer requirements||Intel Core-i7, 8BG RAM, Gigabit Ethernet and Jumbo frame compatible|
|Dimensions without cannula & cables (W x L x H)||8.8 mm x 13.9 mm x 16.6 mm|
|Mass of microscope body excluding cannula & cables||2.2 g|
Single-color Fluorescence Microscope Application NoteDownload (4.24M)
Snap-in Fluorescence Microscope Body & Optogenetically Synchronized Fluorescence Microscope BodyDownload (85.93k)
Snap-in Fluorescence Microscope Body & Optogenetically Synchronized Fluorescence Microscope BodyDownload (91.49k)
For deeper brain regions (150 µm to 8 mm depth) use the Snap-in Imaging Cannula Model L with image guiding gradient-index rod lens that brings the image from inside the brain to the skull surface. Snap-in Imaging Cannulas are compatible with all Fluorescence Microscope Bodies of the corresponding model.
This driver has been designed for the Basic Snap-in and the Optogenetically Synchronized Fluorescence Microscope Bodies.
For in vitro and head-fixed in vivo observations, it is desirable to have a microscope holder coaxial with microscope that fits stereotaxic instrumentation or micromanipulators.
This tool is used to easily attach and detach the Basic Snap-in and the OSFM Microscope Body from the cannula.
For areas near the brain surface (<150 µm depth) use the Snap-in Imaging Cannula Model S. Snap-in Imaging Cannulas are compatible with all Fluorescence Microscope Bodies of the corresponding model.
Our vast product line allows you to assemble systems for miniaturized fluorescence microscopy, fiber pho-tometry and optogenetically synchronized electrophysiology. The Doric Neuroscience Studio is an intuitive software which allows you to control the hardware and acquire all the required data simultaneously.Downloads DORIC STUDIO V188.8.131.52 (72MB)