Optogenetics and electrophysiology are combined in this system to allow recordings of synaptic events at a single-cell resolution in a head-fixed configuration.
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Optogenetics and electrophysiology are combined in this system to allow recordings of synaptic events at a single-cell resolution in a head-fixed configuration. It is designed to activate optogenetic proteins such as channelrhodopsine or to inhibit light-gated ion pumps like halorhodopsin. By adjusting the illumination output power, it is possible to illuminate a single cell or a group of neurons. Extracellular electrophysiology is possible by filling the fiber probe hollow core with an electrolyte solution to get an electrode impedance in the range of 1 to 20 M, allowing a single-unit or multiunit spikes detection.
This system includes specifically:
- Connectorized LED (1x or 2x)
- LED Driver
- Single-cell Opto-electric Probe Holder
- Clamp for Single-cell Opto-electric Probe Holder
- Single-cell Opto-electric Probe Adapter
- Single-cell Opto-electric Probe Interconnect Wire (5x)
- Single-cell Opto-electric Probe Tips (20x)
- Extracellular Electrophysiology Recording System (under development)
- All electrical cables
Doric programmable LED Drivers are available in 1-, 2-, and 4-channel versions. When connected to a Connectorized LED having an eprom memory, the LED Driver recognizes the LED wavelength and automatically sets the maximum current value to avoid accidental overdriving.
Doric Connectorized LEDs couple high brightness LEDs into an FC receptacle compatible with an FC connectorized fiber-optic patch cord. Each Connectorized LED is actively aligned for optimum output power and its optical design provides the maximum fiber-coupling efficiency into multimode optical fibers.
The Single-cell Recording Opto-electric Probe Holder consists of a fiber-optic patch cord in a rigid tubing. It offers an appropriate optical connection between the probe core in the Single-cell Opto-electric Probe Adapter and the light module.
A Single-cell Recording Opto-electric Probe Tip is a piece of dual core optical fiber with a 500 µm optical core and a 250 µm hollow core for electrolyte filling with one end pulled and cut to a 10 µm diameter tip as illustrated above.
The link between the Single-cell Opto-electric Probe Holder and the Single-cell Opto-electric Probe Tip is assured by the Single-cell Opto-electric Probe Adapter. This junction component maintains and aligns the two optical cores together allowing a maximum amount of light to be guided towards the tip.
The Single-cell Opto-electric Interconnect Wire makes the connection between the electrolyte solution within the optical fiber hollow core and the electrophysiology system.
Silica raw dual core fiber in lengths of 55 mm are available for proficient users who prefer to pull and cut the tip by themselves. Suitable with Sutter Instrument P-2000 Laser-based micropipette puller.