Single-cell Optogenetic Illumination and Electrophysiology Recording System

SCRS-OE

Optogenetics and electrophysiology are combined in this system to allow recordings of synaptic events at a single-cell resolution in a head-fixed configuration.

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Optogenetics and electrophysiology are combined in this system to allow recordings of synaptic events at a single-cell resolution in a head-fixed configuration. It is designed to activate optogenetic proteins such as channelrhodopsine or to inhibit light-gated ion pumps like halorhodopsin. By adjusting the illumination output power, it is possible to illuminate a single cell or a group of neurons. Extracellular electrophysiology is possible by filling the fiber probe hollow core with an electrolyte solution to get an electrode impedance in the range of 1 to 20 M, allowing a single-unit or multiunit spikes detection.

This system includes specifically:

- Connectorized LED (1x or 2x)
- LED Driver
- Single-cell Opto-electric Probe Holder
- Clamp for Single-cell Opto-electric Probe Holder
- Single-cell Opto-electric Probe Adapter
- Single-cell Opto-electric Probe Interconnect Wire (5x)
- Single-cell Opto-electric Probe Tips (20x)
- Extracellular Electrophysiology Recording System (under development)
- All electrical cables

  • CAD$0.00

    Doric programmable LED Drivers are available in 1-, 2-, and 4-channel versions. When connected to a Connectorized LED having an eprom memory, the LED Driver recognizes the LED wavelength and automatically sets the maximum current value to avoid accidental overdriving.

    CAD$0.00
  • CAD$0.00

    Doric Connectorized LEDs couple high brightness LEDs into an FC receptacle compatible with an FC connectorized fiber-optic patch cord. Each Connectorized LED is actively aligned for optimum output power and its optical design provides the maximum fiber-coupling efficiency into multimode optical fibers.

    CAD$0.00
  • The Single-cell Recording Opto-electric Probe Holder consists of a fiber-optic patch cord in a rigid tubing. It offers an appropriate optical connection between the probe core in the Single-cell Opto-electric Probe Adapter and the light module.

  • CAD$0.00

    A Single-cell Recording Opto-electric Probe Tip is a piece of dual core optical fiber with a 500 µm optical core and a 250 µm hollow core for electrolyte filling with one end pulled and cut to a 10 µm diameter tip as illustrated above.

    CAD$0.00
  • CAD$0.00

    The link between the Single-cell Opto-electric Probe Holder and the Single-cell Opto-electric Probe Tip is assured by the Single-cell Opto-electric Probe Adapter. This junction component maintains and aligns the two optical cores together allowing a maximum amount of light to be guided towards the tip.

    CAD$0.00
  • CAD$0.00

    The Single-cell Opto-electric Interconnect Wire makes the connection between the electrolyte solution within the optical fiber hollow core and the electrophysiology system. 

    CAD$0.00
  • CAD$0.00

    Silica raw dual core fiber in lengths of 55 mm are available for proficient users who prefer to pull and cut the tip by themselves. Suitable with Sutter Instrument P-2000 Laser-based micropipette puller.

    CAD$0.00