Fiber Photometry System - Single channel Two Colors - GCaMP recording with two excitation wavelengths - 405 and 465 nm

This Single-channel Two-color Fiber Photometry System measures the 405 nm (isosbestic point) excited GCaMP fluorescence, and the 465 nm excited calcium-dependent GCaMP fluorescence, on a single photodetector.

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This Single-channel Two-color Fiber Photometry System measures the 405 nm (isosbestic point) excited GCaMP fluorescence, and the 465 nm excited calcium-dependent GCaMP fluorescence, on a single photodetector. The fluorescence emission can be demodulated by lock-in detection, or by sequential acquisition.

The Single-channel Two-color Fiber Photometry System for GCaMP recording with two excitations wavelengths - 405 and 465 nm contains:

  • LED Fiber Light Sources (405 nm and 465 nm)
  • 2-channel LED Driver
  • Fluorescence Mini Cube with 4 ports - Locked-in or Sequential Detection of Autofluorescence and Fluorophore Excitation (filter set optimized for 405 nm excitation and GFP)
  • Pigtailed 1x1 Fiber-optic Rotary Joint
  • 1x1 Fiber-optic Rotary Joint Holder
  • Photoreceiver Module
  • Fiber-optic Cannulas (10x)
  • Fiber Photometry Cannula Holder
  • Fiber Photometry Console for data acquisition and illumination control
  • Doric Neuroscience Studio Software
  • Optical Breadboard for Connectorized LED
  • Fiber Photometry Rack to mount the whole system
  • All required electrical cables and optical patch cords



Note: Other light sources, i.e. laser diodes or Ce:YAG light sources, and different fluorophore combinations are possible. Please do not hesitate to add your preferences.

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    Doric Connectorized LEDs couple high brightness LEDs into an FC receptacle compatible with an FC connectorized fiber-optic patch cord. Each Connectorized LED is actively aligned for optimum output power and its optical design provides the maximum fiber-coupling efficiency into multimode optical fibers.

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    The Mono Fiber-optic Cannula is an assembly of a bare optical fiber, a fiber ferrule and a receptacle or a sleeve.###: Correspond to the fiber length specified in the ordering code generator section.

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    This FPGA based data acquisition unit synchronizes the output control and the acquisition of the input data. This device seamlessly integrates with Doric Neuroscience Studio, which provides the user-interface for multi-channel photometry experiments.

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  • Our vast product line allows you to assemble systems for miniaturized fluorescence microscopy, fiber pho-tometry and optogenetically synchronized electrophysiology. The Doric Neuroscience Studio is an intuitive software which allows you to control the hardware and acquire all the required data simultaneously.Downloads DORIC STUDIO V5.1.3.1-BETA...

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    An Optical Breadboard for Connectorized LED is available to mount systems including two Connectorized LEDs.

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    Fiber photometry experiments detect small power variations from a fluorophore and for that reason the fiber-optic rotary joints within the setup require minimal transmission variation. 

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    The Fiber Photometry Cannula Holder is designed to enable the recording of the fluorescence during the implantation of the cannula.

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