This Single-channel Two-color Fiber Photometry System measures the 405 nm (isosbestic point) excited GCaMP fluorescence, and the 465 nm excited calcium-dependent GCaMP fluorescence, on a single photodetector.
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This Single-channel Two-color Fiber Photometry System measures the 405 nm (isosbestic point) excited GCaMP fluorescence, and the 465 nm excited calcium-dependent GCaMP fluorescence, on a single photodetector. The fluorescence emission can be demodulated by lock-in detection, or by sequential acquisition.
The Single-channel Two-color Fiber Photometry System for GCaMP recording with two excitations wavelengths - 405 and 465 nm contains:
Note: Other light sources, i.e. laser diodes or Ce:YAG light sources, and different fluorophore combinations are possible. Please do not hesitate to add your preferences.
Doric programmable LED Drivers are available in 1-, 2-, and 4-channel versions. When connected to a Connectorized LED having an eprom memory, the LED Driver recognizes the LED wavelength and automatically sets the maximum current value to avoid accidental overdriving.
Doric Connectorized LEDs couple high brightness LEDs into an FC receptacle compatible with an FC connectorized fiber-optic patch cord. Each Connectorized LED is actively aligned for optimum output power and its optical design provides the maximum fiber-coupling efficiency into multimode optical fibers.
The Mono Fiber-optic Cannula is an assembly of a bare optical fiber, a fiber ferrule and a receptacle or a sleeve.###: Correspond to the fiber length specified in the ordering code generator section.
This FPGA based data acquisition unit synchronizes the output control and the acquisition of the input data. This device seamlessly integrates with Doric Neuroscience Studio, which provides the user-interface for multi-channel photometry experiments.
This version of the FMC4 allows for locked-in or sequential detection of autofluorescence and fluorophore excitation
When used in conjunction with a modulated light source and a lock-in amplifier to reduce the measurement bandwidth, the Newport Photoreceiver Module achieves sensitivity levels in the femtowatt range.
Add behavior tracking to any Doric system with the Doric Color and B&W cameras. The camera uses GigE Vision interface standard typical of high-performance industrial cameras. This interface provides a framework for streaming high-speed video and related control data over Ethernet networks.
Our vast product line allows you to assemble systems for miniaturized fluorescence microscopy, fiber pho-tometry and optogenetically synchronized electrophysiology. The Doric Neuroscience Studio is an intuitive software which allows you to control the hardware and acquire all the required data simultaneously.Downloads DORIC STUDIO V22.214.171.124-BETA...
An Optical Breadboard for Connectorized LED is available to mount systems including two Connectorized LEDs.
Fiber photometry experiments detect small power variations from a fluorophore and for that reason the fiber-optic rotary joints within the setup require minimal transmission variation.
This Fiber Photometry Rack allows for a convenient disposition of all the photometry system parts.
The Fiber Photometry Cannula Holder is designed to enable the recording of the fluorescence during the implantation of the cannula.