This Single-channel Two-color Fiber Photometry System measures the background autofluorescence produced by a 405 nm excitation wavelength and the fluorescence of targeted GFP-like fluorophores produced by a 465 nm excitation wavelength.
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This Single-channel Two-color Fiber Photometry System measures the background autofluorescence produced by a 405 nm excitation wavelength and the fluorescence of targeted GFP-like fluorophores produced by a 465 nm excitation wavelength. The fluorescence emission can be collected with one photodetector and subsequently demodulated or, after spectral separation, collected with respective photodetectors.
The Single-channel Two-color Fiber Photometry System for GFP-like fluorophores contains:
The 1x1 Fiber-optic Rotary Joint is the basic and the most popular type of rotary joints. It can either transmit the light from the sources to the sample and/or from the sample to a photodetector.
This FPGA based data acquisition unit synchronizes the output control and the acquisition of the input data. This device seamlessly integrates with Doric Neuroscience Studio, which provides the user-interface for multi-channel photometry experiments.
The autofluorescence excited by 405 nm light is measure in the 420-450 nm spectral window. In the same time, GCaMP is excited with 460-490 nm light and its fluorescence measured in 500-550 nm spectral window.
When used in conjunction with a modulated light source and a lock-in amplifier to reduce the measurement bandwidth, the Newport Photoreceiver Module achieves sensitivity levels in the femtowatt range.
Add behavior tracking to any Doric system with the Doric Color and B&W cameras. The camera uses GigE Vision interface standard typical of high-performance industrial cameras. This interface provides a framework for streaming high-speed video and related control data over Ethernet networks.
Our vast product line allows you to build different applications such as miniaturized fluorescence microscopy, fiber pho-tometry or optogenetically synchronized electrophysiology. In order to help you implementing the best applications, we created an intuitive software which allows you to control the hardware and acquire all the required...