This photodetector system is designed specifically for use in fiber photometry experiments. Its high gain and low noise allow it to detect signals in the sub-picowatt to nanowatt range. When used with a modulated light source and a lock-in amplifier it can detect signals in the femtowatt range.
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This photodetector system is designed specifically for use in fiber photometry experiments. Its high gain and low noise allow it to detect signals in the sub-picowatt to nanowatt range. When used with a modulated light source and a lock-in amplifier it can detect signals in the femtowatt range. While the detector is normaly integrated into a Fluorescence Mini Cube, it can be used separately with an FC adapter. The photodetector is separated into two parts.
When the detector is not integrated into the iFMC, the Fluorescence Detector Head is fitted with an FC adapter to connect it to a signal source. The detector uses a specialized shielded cable to connect to the amplifier, keeping noise to a minimum.
The Fluorescence Detector Amplifier amplifies the signal coming from the detector head and transmits it to a recording system using a BNC output. The amplifier is wall-mounted, and no battery is required. The amplifier has two detection modes (AC/DC), each selectable for all three (1x, 10x, 100x) amplification levels, allowing 6 different amplification configurations. The DC detection mode is optimal for use with interleaved measurements or during large-bandwidth continuous measurements. The AC detection mode is optimal for lock-in modulation/demodulation and the elimination of low-frequency noise. Its output analog voltage (±5 V) can be monitored with a DAQ board for data recording or an oscilloscope for live signal.
|Amplification level||1x |
|Transimpedance gain||2E9 |
|NEP||less than 12 fW/√Hz|
|Wavelength range||320-1100 nm|
|Peak Sensitivity Wavelength||960 nm|
|Output impedance||50 Ω|
|Bandwidth||DC-1000 Hz ; AC: 30-1000 Hz|
|Detector Size||1.1 x 1.1 (mm x mm)|
User Manual for Doric Fluorescence Detector and Fluorescence Mini Cube with Built-in Detector HeadDownload (3.12M)
Single excitation band fiber photometry measurements use a Fluorescence Mini Cube with 3 ports, with one port for the excitation light, one for the fluorescence detection and one for the sample being tested.
This version of the FMC4 allows for one fluorophore excitation and detection, and one opsin activation
The GCaMP isosbestic point is excited by the 400-410 nm bandwidth light and the 460-490 nm spectral window excites its functional fluorescence.
This version of the FMC4 allows for locked-in or sequential detection of isosbestic and functional excitations.
This version of the FMC4 is comprised of one fluorescence excitation channel, and two detection channels.
This cube is used for green and red fluorophore excitation and their respective detection wavelengths. Other fluorophore combinations are possible.
This cube can be used to detect the fluorescence from two calcium indicators and the GCaMP isosbestic point.
This mini-cube separates three different indicators simultaneously.