This cube permits excitation of the GCaMP isosbestic point with 400-410 nm light and fluorophores with 460-490 nm light. The single detector measures both signals within the fluorescence detection window from 500-550 nm.
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In order to optimize signal detection and simplify usage, it is desirable to minimize the number of fiber optic connections to a Fluorescence Mini Cube. By taking advantages of the new Doric Fluorescence Detector, and our Built-in LED Optical Heads, it is possible to have an Integrated Fluorescence Mini Cube.
This cube permits excitation of the GCaMP isosbestic point with 400-410 nm light and fluorophores with 460-490 nm light. The single detector measures both signals within the fluorescence detection window from 500-550 nm. The separation of GCaMP isosbestic and functional excitations is possible if both excitations are modulated.
On the image IE and E are ports for excitations, F is for fluorescence detection and S is the for fixed sample port. The numbers in the brackets of the ordering code denote the corresponding wavelength bands.
|Amplification level||1x |
|Transimpedance gain||2E9 |
|Coupling efficiency||Over 75% in the bandwidth of interest|
|NEP||less than 12 fW/√Hz|
|Optical filter attenuation||Over OD 5 outside the bandwidth of interest|
|Wavelength range||320-1100 nm|
|Peak Sensitivity Wavelength||960 nm|
|Typical spectral bandwidth||Excitation: 400-410 nm, 460-490 nm; Emission: 500-550 nm|
|Output impedance||50 Ω|
|Bandwidth||DC-1000 Hz ; AC: 30-1000 Hz|
|Dimensions||111 mm x 114 mm (Depth x Width)|
|Detector Size||1.1 x 1.1 (mm x mm)|
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