Fluorescence Mini-cube with 4 ports - Locked-in or Sequential Detection for GCaMP Isosbestic and Functional Excitations

This version of the FMC4 allows for locked-in or sequential detection of isosbestic and functional excitations.

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This cube permits excitation of the GCaMP isosbestic point with 400-410 nm light and fluorophores with 460-490 nm light. The single detector measures both signals within the fluorescence detection window from 500-550 nm. The separation of GCaMP isosbestic and functional excitations is possible if both excitations are modulated.

On the image IE and E are ports for excitations, F is for fluorescence detection and S is the for fixed sample port. The numbers in the brackets of the ordering code denote the corresponding wavelength bands.

Coupling efficiencyOver 75% in the bandwidth of interest
Optical filter attenuationOver OD 5 outside the bandwidth of interest
Typical spectral bandwidthExcitation: 400-410 nm, 460-490 nm; Emission: 500-550 nm
Typical fiber configurationExcitation and Sample: 200 or 400 µm core, NA 0.48 or 0.53; Detection: 600 µm core, NA 0.48
Dimensions76 mm x 67 mm x 26 mm including connectors

FMC4-DRAWING.pdf

4-port fluorescence mini cube dimensions

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