This version of the FMC4 allows for locked-in or sequential detection of isosbestic and functional excitations.
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This cube permits excitation of the GCaMP isosbestic point with 400-410 nm light and fluorophores with 460-490 nm light. The single detector measures both signals within the fluorescence detection window from 500-550 nm. The separation of GCaMP isosbestic and functional excitations is possible if both excitations are modulated.
On the image IE and E are ports for excitations, F is for fluorescence detection and S is the for fixed sample port. The numbers in the brackets of the ordering code denote the corresponding wavelength bands.
|Coupling efficiency||Over 75% in the bandwidth of interest|
|Optical filter attenuation||Over OD 5 outside the bandwidth of interest|
|Typical spectral bandwidth||Excitation: 400-410 nm, 460-490 nm; Emission: 500-550 nm|
|Typical fiber configuration||Excitation and Sample: 200 or 400 µm core, NA 0.48 or 0.53; Detection: 600 µm core, NA 0.48|
|Dimensions||76 mm x 67 mm x 26 mm including connectors|
This FPGA based data acquisition unit synchronizes the output control and the acquisition of the input data. This device seamlessly integrates with Doric Neuroscience Studio, which provides the user-interface for multi-channel photometry experiments.
Optical fiber patch cords with an integrated attenuating filter are ideal for applications where optical power coupled into a fiber is too high, i.e. fiber photometry excitation.
This photodetector system is designed specifically for use in fiber photometry experiments. Its high gain and low noise allow it to detect signals in the sub-picowatt to nanowatt range. When used with a modulated light source and a lock-in amplifier it can detect signals in the femtowatt range.
The Low Autofluorescence Mono Patch Cords are specially designed to minimize the fluorescence caused by photoactive compounds in the optical fiber and associated elements.