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Fluorescence Mini-cube with 4 ports - Locked-in or Sequential Detection for GCaMP Isosbestic and Functional Excitations
This version of the FMC4 allows for locked-in or sequential detection of isosbestic and functional excitations.
This cube permits excitation of the GCaMP isosbestic point with 400-410 nm light and fluorophores with 460-490 nm light. The single detector measures both signals within the fluorescence detection window from 500-550 nm. The separation of GCaMP isosbestic and functional excitations is possible if both excitations are modulated.
On the image IE and E are ports for excitations, F is for fluorescence detection and S is the for fixed sample port. The numbers in the brackets of the ordering code denote the corresponding wavelength bands.
| Coupling efficiency | Over 75% in the bandwidth of interest |
| Optical filter attenuation | Over OD 5 outside the bandwidth of interest |
| Typical spectral bandwidth | Excitation: 400-410 nm, 460-490 nm; Emission: 500-550 nm |
| Typical fiber configuration | Excitation and Sample: 200 or 400 µm core, NA 0.48 or 0.53; Detection: 600 µm core, NA 0.48 |
| Dimensions | 76 mm x 67 mm x 26 mm including connectors |
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