This cube permits excitation of the GCaMP isosbestic point with 400-410nm light and ﬂuorophores with 460-490nm light. The camera measures both signals within the ﬂuorescence detection window from 500-550nm.The separation of GCaMP isosbestic and functional excitations is possible if both excitations are interleaved.
This cube can be used to detect the fluorescence from two calcium indicators and the GCaMP isosbestic point. The separation of functional and isosbestic fluorescence signals of GCaMP is possible if the light sources are interleaved. This cube contains two cameras, one for each fluorescence port.